Tumors carrying BRAF-mutations over-express NAMPT that is genetically amplified and possesses oncogenic properties.

ABSTRACT:

Background

Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in nicotinamide adenine dinucleotide (NAD) biosynthesis, is up-regulated in several cancers, including metastatic melanoma (MM). The BRAF oncogene is mutated in different cancer types, among which MM and thyroid carcinoma (THCA) are prominent. Drugs targeting mutant BRAF are effective, especially in MM patients, even though resistance rapidly develops. Previous data have linked NAMPT over-expression to the acquisition of BRAF resistance, paving the way for therapeutic strategies targeting the two pathways.

Methods

Exploiting the TCGA database and a collection of MM and THCA tissue microarrays we studied the association between BRAF mutations and NAMPT expression. BRAF wild-type (wt) cell line

SUBMITTER: Audrito V 

PROVIDER: S-EPMC8908704 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature