Maintenance of Chronological Aging Features in Culture of Normal Human Dermal Fibroblasts from Old Donors.

ABSTRACT: Chronological aging is defined as a time-dependent decline of tissue homeostasis which severely impacts skin. Understanding the mechanisms of skin aging is an active research area limited by the lack of relevant in vitro models. Being a component of aging, replicative or stress-induced senescence is repeatedly used to mimic skin aging in vitro, thus presenting only a partial view of the complexity of aging. Herein, we aimed to clarify whether primary normal human dermal fibroblasts retained age-related characteristics when cultured in 2D monolayer, and could be used as a relevant model for aging research. We compared three groups of fibroblasts isolated from different aged donors. We observed strongly decreased population doubling capacities, a reduced clonogenic ability, an impairment in

SUBMITTER: Rorteau J 

PROVIDER: S-EPMC8909060 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature