Pyk2 Regulates MAMs and Mitochondrial Dynamics in Hippocampal Neurons.

ABSTRACT: Pyk2 is a non-receptor tyrosine kinase enriched in hippocampal neurons, which can be activated by calcium-dependent mechanisms. In neurons, Pyk2 is mostly localised in the cytosol and dendritic shafts but can translocate to spines and/or to the nucleus. Here, we explore the function of a new localisation of Pyk2 in mitochondria-associated membranes (MAMs), a subdomain of ER-mitochondria surface that acts as a signalling hub in calcium regulation. To test the role of Pyk2 in MAMs' calcium transport, we used full Pyk2 knockout mice (Pyk2^-/-) for in vivo and in vitro studies. Here we report that Pyk2^-/- hippocampal neurons present increased ER-mitochondrial contacts along with defective calcium homeostasis. We also show how the absence of Pyk2 modulates mitochondrial dyn

SUBMITTER: Lopez-Molina L 

PROVIDER: S-EPMC8909471 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature