Functional Ex Vivo Tissue-Based Chemotherapy Sensitivity Testing for Breast Cancer.

ABSTRACT: Background chemotherapy is part of most breast cancer (BC) treatment schedules. However, a substantial fraction of BC tumors does not respond to the treatment. Unfortunately, no standard biomarkers exist for response prediction. Therefore, we aim to develop ex vivo sensitivity assays for two types of commonly used cytostatics (i.e., platinum derivates and taxanes) on organotypic BC tissue slices. Ex vivo cisplatin sensitivity assays were established using organotypic tissue slices derived from the surgical resection material of 13 primary BCs and 20 fresh histological biopsies obtained from various metastatic sites. Furthermore, tissue slices of 10 primary BCs were used to establish a docetaxel ex vivo sensitivity assay. Cisplatin sensitivity was assessed by tissue morphology, proliferatio

SUBMITTER: Ladan MM 

PROVIDER: S-EPMC8909506 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature