Dynamics of Endothelial Engagement and Filopodia Formation in Complex 3D Microscaffolds.

ABSTRACT: The understanding of endothelium-extracellular matrix interactions during the initiation of new blood vessels is of great medical importance; however, the mechanobiological principles governing endothelial protrusive behaviours in 3D microtopographies remain imperfectly understood. In blood capillaries submitted to angiogenic factors (such as vascular endothelial growth factor, VEGF), endothelial cells can transiently transdifferentiate in filopodia-rich cells, named tip cells, from which angiogenesis processes are locally initiated. This protrusive state based on filopodia dynamics contrasts with the lamellipodia-based endothelial cell migration on 2D substrates. Using two-photon polymerization, we generated 3D microstructures triggering endothelial phenotypes evocative of tip cell behavi

SUBMITTER: Ucla P 

PROVIDER: S-EPMC8910162 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature