Genetic Dissection of CRISPR-Cas9 Mediated Inheritance of Independently Targeted Alleles in Tobacco α-1,3-Fucosyltransferase 1 and β-1,2-Xylosyltransferase 1 Loci.

ABSTRACT: We determined the specificity of mutations induced by the CRISPR-Cas9 gene-editing system in tobacco (Nicotiana benthamiana) alleles and subsequent genetic stability. For this, we prepared 248 mutant plants using an Agrobacterium-delivered CRISPR-Cas9 system targeting α-1,3-fucosyltransferase 1 (FucT1) and β-1,2-xylosyltransferase1 (XylT1) genes, for which the mutation rates were 22.5% and 25%, respectively, with 20.5% for both loci. Individuals with wild-type (WT) alleles at the NbFucT1 locus in T₀ were further segregated into chimeric progeny (37-54%) in the next generation, whereas homozygous T₀ mutants tended to produce more (~70%) homozygotes than other bi-allelic and chimeric progenies in the T₁

SUBMITTER: Song H 

PROVIDER: S-EPMC8910323 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature