Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells.

ABSTRACT: Both the dental pulp and the apical papilla represent a promising source of mesenchymal stem cells for regenerative endodontic protocols. The aim of this study was to outline molecular biological conformities and differences between dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). Thus, cells were isolated from the pulp and the apical papilla of an extracted molar and analyzed for mesenchymal stem cell markers as well as multi-lineage differentiation. During induced osteogenic differentiation, viability, proliferation, and wound healing assays were performed, and secreted signaling molecules were quantified by enzyme-linked immunosorbent assays (ELISA). Transcriptome-wide gene expression was profiled by microarrays and validated by quantitative reverse transcrip

SUBMITTER: Smeda M 

PROVIDER: S-EPMC8910327 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature