Production of an Active, Human Membrane Protein in Saccharomyces cerevisiae: Full-Length FICD.

ABSTRACT: The human Fic domain-containing protein (FICD) is a type II endoplasmic reticulum (ER) membrane protein that is important for the maintenance of ER proteostasis. Structural and in vitro biochemical characterisation of FICD AMPylase and deAMPylase activity have been restricted to the soluble ER-luminal domain produced in Escherichia coli. Information about potentially important features, such as structural motifs, modulator binding sites or other regulatory elements, is therefore missing for the approximately 100 N-terminal residues including the transmembrane region of FICD. Expressing and purifying the required quantity and quality of membrane proteins is demanding because of the low yields and poor stability often observed. Here, we produce full-length FICD by combining a Sacch

SUBMITTER: Virolainen MS 

PROVIDER: S-EPMC8910494 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature