Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats.

ABSTRACT: Skeletal muscles are an important reservoir of nitric oxide (NO^•) stored in the form of nitrite [NO₂^-] and nitrate [NO₃^-] (NO_x). Nitrite, which can be reduced to NO^• under hypoxic and acidotic conditions, is considered a physiologically relevant, direct source of bioactive NO^•. The aim of the present study was to determine the basal levels of NO_x in striated muscles (including rat heart and locomotory muscles) with varied contents of tissue nitrite reductases, such as myoglobin and mitochondrial electron transport chain proteins (ETC-proteins). Muscle NO_x was determined using a high-performance liquid chromatography-based method. Muscle proteins were evaluated using western-immunob

SUBMITTER: Majerczak J 

PROVIDER: S-EPMC8910716 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature