Protoplast Dissociation and Transcriptome Analysis Provides Insights to Salt Stress Response in Cotton.

ABSTRACT: As one of the pioneer crops widely planted in saline-alkaline areas, Gossypium provides daily necessities, including natural fiber, vegetable proteins, and edible oils. However, cotton fiber yield and quality are highly influenced by salt stress. Therefore, elucidating the molecular mechanisms of cotton in response to salinity stress is importance to breed new cultivars with high tolerance. In this study, we first developed a method for single-cell RNA-seq based on isolating protoplast from cotton root tips; then, we studied the impact of salinity stress on gene expression profiling and their dynamic changes using the developed high-efficiency method for protoplast dissociation suitable for single-cell RNA-seq. A total of 3391 and 2826 differentially expressed genes (DEGs) were iden

SUBMITTER: Liu Q 

PROVIDER: S-EPMC8911145 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature