Ontology highlight
ABSTRACT:
SUBMITTER: Sampathkumar NK
PROVIDER: S-EPMC8912902 | biostudies-literature | 2022 Feb
REPOSITORIES: biostudies-literature
Sampathkumar Nirmal Kumar NK Sundaram Venkat Krishnan VK Danthi Prakroothi S PS Barakat Rasha R Solomon Shiden S Mondal Mrityunjoy M Carre Ivo I El Jalkh Tatiana T Padilla-Ferrer Aïda A Grenier Julien J Massaad Charbel C Mitchell Jacqueline C JC
PLoS computational biology 20220228 2
Assessment of differential gene expression by qPCR is heavily influenced by the choice of reference genes. Although numerous statistical approaches have been proposed to determine the best reference genes, they can give rise to conflicting results depending on experimental conditions. Hence, recent studies propose the use of RNA-Seq to identify stable genes followed by the application of different statistical approaches to determine the best set of reference genes for qPCR data normalization. In ...[more]