TIGER: inferring DNA replication timing from whole-genome sequence data.

ABSTRACT:

Motivation

Genomic DNA replicates according to a reproducible spatiotemporal program, with some loci replicating early in S phase while others replicate late. Despite being a central cellular process, DNA replication timing studies have been limited in scale due to technical challenges.

Results

We present TIGER (Timing Inferred from Genome Replication), a computational approach for extracting DNA replication timing information from whole genome sequence data obtained from proliferating cell samples. The presence of replicating cells in a biological specimen leads to non-uniform representation of genomic DNA that depends on the timing of replication of different genomic loci. Replication dynamics can hence be observed in genome sequence data by analyzing DNA copy number along

SUBMITTER: Koren A 

PROVIDER: S-EPMC8913259 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature