Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironment.

ABSTRACT: We investigated the role of the NFE2L3 transcription factor in inflammation-induced colorectal cancer. Our studies revealed that Nfe2l3^-/- mice exhibit significantly less inflammation in the colon, reduced tumor size and numbers, and skewed localization of tumors with a more pronounced decrease of tumors in the distal colon. CIBERSORT analysis of RNA-seq data from normal and tumor tissue predicted a reduction in mast cells in Nfe2l3^-/- animals, which was confirmed by toluidine blue staining. Concomitantly, the transcript levels of Il33 and Rab27a, both important regulators of mast cells, were reduced and increased, respectively, in the colorectal tumors of Nfe2l3^-/- mice. Furthermore, we validated NFE2L3 binding to the regulatory sequences of the IL33 and R

SUBMITTER: Saliba J 

PROVIDER: S-EPMC8913363 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature