Atomic force microscopy-single-molecule force spectroscopy unveils GPCR cell surface architecture.

ABSTRACT: G protein-coupled receptors (GPCRs) form the largest family of cell surface receptors. Despite considerable insights into their pharmacology, the GPCR architecture at the cell surface still remains largely unexplored. Herein, we present the specific unfolding of different GPCRs at the surface of living mammalian cells by atomic force microscopy-based single molecule force spectroscopy (AFM-SMFS). Mathematical analysis of the GPCR unfolding distances at resting state revealed the presence of different receptor populations relying on distinct oligomeric states which are receptor-specific and receptor expression-dependent. Moreover, we show that the oligomer size dictates the receptor spatial organization with nanoclusters of high-order oligomers while lower-order complexes spread over the wh

SUBMITTER: Dague E 

PROVIDER: S-EPMC8913689 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature