Scanning laser-induced endothelial injury: a standardized and reproducible thrombosis model for intravital microscopy.

ABSTRACT: Vascular injury models are indispensable for studying thrombotic processes in vivo. Amongst the available methods for inducing thrombosis, laser-induced endothelial injury (LIEI) has several unique advantages. However, a lack of methodological standardization and expensive instrumentation remain significant problems decreasing reproducibility and impeding the adoption of LIEI in the wider scientific community. In this, study, we developed a standardized protocol for scanning laser-induced endothelial injury (scanning-LIEI) of murine mesenteric veins using the intrinsic 405 nm laser of a conventional laser scanning confocal microscope. We show that our model produces thrombi with prominent core-shell architectures and minimal radiation-related fluorescence artefacts. In comparison with prev

SUBMITTER: Larsson P 

PROVIDER: S-EPMC8913794 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature