TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4⁺ T-Cell Balance in Allergic Rhinitis.

ABSTRACT:

Purpose

Previous studies have shown the role of ten-eleven translocation 2 (TET2) in CD4⁺ T cells. However, its function in CD4⁺ T cells under allergic inflammation is unclear. We aimed to investigate the epigenomic distribution of DNA 5-hydroxymethylcytosine (5hmC) and the role of TET2 in CD4⁺ T cells of allergic rhinitis (AR).

Methods

The hMeDIP-seq was performed to identify sequences with 5hmC deposition in CD4⁺ T cells of AR patients. Tet2-deficient or wild type mice were stimulated with ovalbumin (OVA) to develop an AR mouse model. The histopathology in nasal mucosae, Th1/Th2/Treg/Th17 cell percentage, concentrations of Th-related cytokines, expression of Tets and differential hydroxymethylated genes (DhMG), and the glo

SUBMITTER: Tan L 

PROVIDER: S-EPMC8914607 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature