Investigating the Performances of Wide-Field Raman Microscopy with Stochastic Optical Reconstruction Post-Processing.

ABSTRACT: Super-resolution fluorescence microscopy based on localization algorithms has tremendously impacted the field of imaging by improving the spatial resolution of optical measurements with specific blinking fluorophores and concomitant reduction of acquisition time. In vibrational spectroscopy and imaging, various methods have been developed to surpass the diffraction limit including near-field scattering methods, such as in tip-enhanced Raman and infrared spectroscopies. Although these scanning-probe techniques can provide exquisite spatial resolution, they often require long acquisition times and tedious fabrication of nano-scale scanning probes. Herein, stochastic optical reconstruction microscopy (STORM) protocol is applied on Raman measurements acquired using a wide-field home-built micr

SUBMITTER: Mazaheri L 

PROVIDER: S-EPMC8915227 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature