Effects of Prenatal Exposure to Titanium Dioxide Nanoparticles on DNA Methylation and Gene Expression Profile in the Mouse Brain.

ABSTRACT: Background and Objectives: Titanium dioxide nanoparticles (TiO₂-NP) are important materials used in commercial practice. Reportedly, TiO₂-NP exposure during pregnancy can affect the development of the central nervous system in mouse offspring; however, the underlying mechanism remains unknown. In the present study, we investigated the impact of prenatal TiO₂-NP exposure on global DNA methylation and mRNA expression patterns in the brains of neonatal mice. Materials and Methods: Pregnant C57BL/6J mice were intratracheally administered a TiO₂-NP suspension (100 μg/mouse) on gestational day 10.5, and brains were collected from male and female offspring at day 1 postpartum. After extraction of methylated DNA by immunoprecipitation, the

SUBMITTER: Tachibana K 

PROVIDER: S-EPMC8915839 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature