Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.

ABSTRACT: The practice of serial X-ray crystallography (SX) depends on efficient, continuous delivery of hydrated protein crystals while minimizing background scattering. Of the two major types of sample delivery devices, fixed-target devices offer several advantages over widely adopted jet injectors, including: lower sample consumption, clog-free delivery, and the ability to control on-chip crystal density to improve hit rates. Here we present our development of versatile, inexpensive, and robust polymer microfluidic chips for routine and reliable room temperature serial measurements at both synchrotrons and X-ray free electron lasers (XFELs). Our design includes highly X-ray-transparent enclosing thin film layers tuned to minimize scatter background, adaptable sample flow layers tuned to match cry

SUBMITTER: Gilbile D 

PROVIDER: S-EPMC8915944 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature