Type I Interferon Response Is Mediated by NLRX1-cGAS-STING Signaling in Brain Injury.

ABSTRACT:

Background

Inflammation is a significant contributor to neuronal death and dysfunction following traumatic brain injury (TBI). Recent evidence suggests that interferons may be a key regulator of this response. Our studies evaluated the role of the Cyclic GMP-AMP Synthase-Stimulator of Interferon Genes (cGAS-STING) signaling pathway in a murine model of TBI.

Methods

Male, 8-week old wildtype, STING knockout (^-/-), cGAS ^-/-, and NLRX1 ^-/- mice were subjected to controlled cortical impact (CCI) or sham injury. Histopathological evaluation of tissue damage was assessed using non-biased stereology, which was complemented by analysis at the mRNA and protein level using qPCR and western blot analysis, respectively.

Results

SUBMITTER: Fritsch LE 

PROVIDER: S-EPMC8916033 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature