The function of chloroplast ferredoxin-NADP⁺ oxidoreductase positively regulates the accumulation of bamboo mosaic virus in Nicotiana benthamiana.

ABSTRACT: A gene down-regulated in Nicotiana benthamiana after bamboo mosaic virus (BaMV) infection had high identity to the nuclear-encoded chloroplast ferredoxin NADP⁺ oxidoreductase gene (NbFNR). NbFNR is a flavoenzyme involved in the photosynthesis electron transport chain, catalysing the conversion of NADP⁺ into NADPH. To investigate whether NbFNR is involved in BaMV infection, we used virus-induced gene silencing to reduce the expression of NbFNR in leaves and protoplasts. After BaMV inoculation, the accumulation of BaMV coat protein and RNA was significantly reduced. The transient expression of NbFNR fused with orange fluorescent protein (OFP) localized in the chloroplasts and elevated the level of BaMV coat protein. These results suggest that NbFNR could play a positive

SUBMITTER: Chen IH 

PROVIDER: S-EPMC8916203 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature