An unconventionally secreted effector from the root knot nematode Meloidogyne incognita, Mi-ISC-1, promotes parasitism by disrupting salicylic acid biosynthesis in host plants.

ABSTRACT: Plant-parasitic nematodes need to deliver effectors that suppress host immunity for successful parasitism. We have characterized a novel isochorismatase effector from the root-knot nematode Meloidogyne incognita, named Mi-ISC-1. The Mi-isc-1 gene is expressed in the subventral oesophageal glands and is up-regulated in parasitic-stage juveniles. Tobacco rattle virus-induced gene silencing targeting Mi-isc-1 attenuated M. incognita parasitism. Enzyme activity assays confirmed that Mi-ISC-1 can catalyse hydrolysis of isochorismate into 2,3-dihydro-2,3-dihydroxybenzoate in vitro. Although Mi-ISC-1 lacks a classical signal peptide for secretion at its N-terminus, a yeast invertase secretion assay showed that this protein can be secreted from eukaryotic cells. However, the subcellular localizati

SUBMITTER: Qin X 

PROVIDER: S-EPMC8916211 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature