ALKBH3-dependent m¹A demethylation of Aurora A mRNA inhibits ciliogenesis.

ABSTRACT: Primary cilia are antenna-like subcellular structures to act as signaling platforms to regulate many cellular processes and embryonic development. m¹A RNA modification plays key roles in RNA metabolism and gene expression; however, the physiological function of m¹A modification remains largely unknown. Here we find that the m¹A demethylase ALKBH3 significantly inhibits ciliogenesis in mammalian cells by its demethylation activity. Mechanistically, ALKBH3 removes m¹A sites on mRNA of Aurora A, a master suppressor of ciliogenesis. Depletion of ALKBH3 enhances Aurora A mRNA decay and inhibits its translation. Moreover, alkbh3 morphants exhibit ciliary defects, including curved body, pericardial edema, abnormal otoliths, and dilation in pronephric du

SUBMITTER: Kuang W 

PROVIDER: S-EPMC8917145 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature