Chemical genetics of regeneration: Contrasting temporal effects of CoCl₂ on axolotl tail regeneration.

ABSTRACT:

Background

Histone deacetylases (HDACs) regulate transcriptional responses to injury stimuli that are critical for successful tissue regeneration. Previously we showed that HDAC inhibitor romidepsin potently inhibits axolotl tail regeneration when applied for only 1-minute postamputation (MPA).

Results

Here we tested CoCl_2, a chemical that induces hypoxia and cellular stress, for potential to reverse romidepsin inhibition of tail regeneration. Partial rescue of regeneration was observed among embryos co-treated with romidepsin and CoCl₂ for 1 MPA, however, extending the CoCl₂ dosage window either inhibited regeneration (CoCl₂ :0 to 30 MPA) or was lethal (CoCl₂ :0 to 24 hours postamputation; HPA). CoCl₂ :0 to 3

SUBMITTER: Baddar NWAH 

PROVIDER: S-EPMC8917379 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature