Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells.

ABSTRACT:

Background

Cancer stem cells (CSCs) are thought to play important roles in carcinogenesis, recurrence, metastasis, and therapy-resistance. We have successfully induced cancer stem-like sphere cells (CSLCs) which possess enhanced chemoresistance and metastatic potential. To enable the development of targeted therapy against CSLCs, we identified a gene responsible for this phenotype in CSLC.

Methods

Human hepatoma cell line SK-HEP-1 was used for CSLC induction with a unique sphere inducing medium, and HuH-7 cells were used as non-sphere forming cells in the same condition. RNA-sequencing was performed followed by validation with quantitative RT-PCR and western blotting. Knockdown experiments were done by using CRISPR-Cas9 genome-editing, and the rescue experiments were perform

SUBMITTER: Tsunedomi R 

PROVIDER: S-EPMC8917729 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature