Genome-wide investigation of maize RAD51 binding affinity through phage display.

ABSTRACT:

Background

RAD51 proteins, which are conserved in all eukaryotes, repair DNA double-strand breaks. This is critical to homologous chromosome pairing and recombination enabling successful reproduction. Work in Arabidopsis suggests that RAD51 also plays a role in plant defense; the Arabidopsis rad51 mutant is more susceptible to Pseudomonas syringae. However, the defense functions of RAD51 and the proteins interacting with RAD51 have not been thoroughly investigated in maize. Uncovering ligands of RAD51 would help to understand meiotic recombination and possibly the role of RAD51 in defense. This study used phage display, a tool for discovery of protein-protein interactions, to search for proteins interacting with maize RAD51A1.

Results

Maize RAD51A1 was screened against a ran

SUBMITTER: Milsted C 

PROVIDER: S-EPMC8917730 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature