Drug metabolic activity is a critical cell-intrinsic determinant for selection of hepatocytes during long-term culture.

ABSTRACT:

Background

The liver plays an important role in various metabolic processes, including protein synthesis, lipid and drug metabolisms and detoxifications. Primary culture of hepatocytes is used for the understanding of liver physiology as well as for the drug development. Hepatocytes are, however, hardly expandable in vitro making it difficult to secure large numbers of cells from one donor. Alternatively, systems using animal models and hepatocellular carcinoma cells have been established, but interspecies differences, variation between human cell sources and limited hepatic functions are among the challenges faced when using these models. Therefore, there is still a need for a highly stable method to purify human hepatocytes with functional sufficiency. In this study, we aimed to

SUBMITTER: Akiyama S 

PROVIDER: S-EPMC8917760 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature