Impact of linker histone in the formation of ambochlorin-calf thymus DNA complex: Multi-spectroscopic, stopped-flow, and molecular modeling approaches.

ABSTRACT:

Objectives

This study aimed to evaluate the role of the linker histone (H₁) in the binding interaction between ambochlorin (Amb), and calf thymus DNA (ctDNA) as binary and ternary systems.

Materials and methods

The project was accomplished through the means of absorbance, fluorescence, stopped-flow circular dichroism spectroscopy, viscosity, thermal melting, and molecular modeling techniques.

Results

Spectroscopic analysis revealed that although Amb was strongly bound to both ctDNA and ctDNA-H₁, it showed a greater tendency to ctDNA in the presence of the linker histone. The obtained thermodynamic parameters revealed that both Amb-ctDNA and Amb-ctDNA-H₁ interactions were spontaneous, endothermic, and entropy-favored, and hydrophobic in

SUBMITTER: Askari A 

PROVIDER: S-EPMC8917854 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature