3D-printed oxygen-releasing scaffolds improve bone regeneration in mice.

ABSTRACT: Low oxygen (O₂) diffusion into large tissue engineered scaffolds hinders the therapeutic efficacy of transplanted cells. To overcome this, we previously studied hollow, hyperbarically-loaded microtanks (μtanks) to serve as O₂ reservoirs. To adapt these for bone regeneration, we fabricated biodegradable μtanks from polyvinyl alcohol and poly (lactic-co-glycolic acid) and embedded them to form 3D-printed, porous poly-ε-caprolactone (PCL)-μtank scaffolds. PCL-μtank scaffolds were loaded with pure O₂ at 300-500 psi. When placed at atmospheric pressures, the scaffolds released O₂ over a period of up to 8 h. We confirmed the inhibitory effects of hypoxia on the osteogenic differentiation of human adipose-derived stem cells (hASCs and we validated that

SUBMITTER: Farris AL 

PROVIDER: S-EPMC8918039 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature