Subcellular progression of mesenchymal transition identified by two discrete synchronous cell lines derived from the same glioblastoma.

ABSTRACT: Glioblastomas (GBM) exhibit intratumoral heterogeneity of various oncogenic evolutional processes. We have successfully isolated and established two distinct cancer cell lines with different morphological and biological characteristics that were derived from the same tissue sample of a GBM. When we compared their genomic and transcriptomic characteristics, each cell line harbored distinct mutation clusters while sharing core driver mutations. Transcriptomic analysis revealed that one cell line was undergoing a mesenchymal transition process, unlike the other cell line. Furthermore, we could identify four tumor samples containing our cell line-like clusters from the publicly available single-cell RNA-seq data, and in a set of paired longitudinal GBM samples, we could confirm three pairs whe

SUBMITTER: Kim S 

PROVIDER: S-EPMC8918182 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature