The distinctive roles played by the superoxide dismutases of the extremophile Acinetobacter sp. Ver3.

ABSTRACT: Acinetobacter sp. Ver3 is a polyextremophilic strain characterized by a high tolerance to radiation and pro-oxidants. The Ver3 genome comprises the sodB and sodC genes encoding an iron (^AV3SodB) and a copper/zinc superoxide dismutase (^AV3SodC), respectively; however, the specific role(s) of these genes has remained elusive. We show that the expression of sodB remained unaltered in different oxidative stress conditions whereas sodC was up-regulated in the presence of blue light. Besides, we studied the changes in the in vitro activity of each SOD enzyme in response to diverse agents and solved the crystal structure of ^AV3SodB at 1.34 Å, one of the highest resolutions achieved for a SOD. Cell fractionation studies interestingly revealed that ^AV3Sod

SUBMITTER: Steimbruch BA 

PROVIDER: S-EPMC8918354 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature