PET imaging of glycogen synthase kinase-3 in pancreatic cancer xenograft mouse models.

ABSTRACT: Glycogen synthase kinase-3 (GSK-3) contributes to tumorigenesis in pancreatic cancer by modulating cell proliferation and survival. This study evaluated the lead GSK-3 targeted PET radiotracers for neuro-PET imaging, [¹¹C]PF-367 and [¹¹C]OCM-44, in pancreatic cancer xenograft mice. Immunohistochemistry showed that GSK-3α and GSK-3β were overexpressed in PANC-1 xenografts. In autoradiography studies, higher specific binding was observed for [³H]PF-367 compared to [³H]OCM-44 when co-incubated with unlabeled PF-367 (59.2±1.8% vs 22.6±3.75%, respectively). Co-incubation of [¹¹C]OCM-44 with OCM-44 did not improve the specific binding (25.5±30.2%). In dynamic PET imaging of PANC-1 xenograft mouse models, tumors were not visualized with [

SUBMITTER: Boyle AJ 

PROVIDER: S-EPMC8918402 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature