Development of a 3D atlas of the embryonic pancreas for topological and quantitative analysis of heterologous cell interactions.

ABSTRACT: Generating comprehensive image maps, while preserving spatial three-dimensional (3D) context, is essential in order to locate and assess quantitatively specific cellular features and cell-cell interactions during organ development. Despite recent advances in 3D imaging approaches, our current knowledge of the spatial organization of distinct cell types in the embryonic pancreatic tissue is still largely based on two-dimensional histological sections. Here, we present a light-sheet fluorescence microscopy approach to image the pancreas in three dimensions and map tissue interactions at key time points in the mouse embryo. We demonstrate the utility of the approach by providing volumetric data, 3D distribution of three main cellular components (epithelial, mesenchymal and endothelial cells)

SUBMITTER: Glorieux L 

PROVIDER: S-EPMC8918780 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature