The Pharmacological and Structural Basis of the AahII-Na_V1.5 Interaction and Modulation by the Anti-AahII Nb10 Nanobody.

ABSTRACT: Scorpion α-toxins are neurotoxins that target the fast inactivation mechanism of voltage-gated sodium (Na_V) channels leading to several neuro- and cardiotoxic effects in mammals. The toxin AahII is the most active α-toxin from the North African scorpion Androctonus australis Hector that slows the fast inactivation of Na_V channels. To fight scorpion envenomation, an anti-AahII nanobody named NbAahII10 (Nb10) was developed. The efficiency of this nanobody has been evaluated in vivo on mice, but its mechanism of action at the cellular level remains unknown. Here we have shown that AahII toxin slows the fast inactivation of the adult cardiac Na_V1.5 channels, expressed in HEK293 cells, in a dose-dependent manner, while current amplitude was not aff

SUBMITTER: Hmaidi R 

PROVIDER: S-EPMC8918821 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature