ADAD2 regulates heterochromatin in meiotic and post-meiotic male germ cells via translation of MDC1.

ABSTRACT: Male germ cells establish a unique heterochromatin domain, the XY-body, early in meiosis. How this domain is maintained through the end of meiosis and into post-meiotic germ cell differentiation is poorly understood. ADAD2 is a late meiotic male germ cell-specific RNA-binding protein, loss of which leads to post-meiotic germ cell defects. Analysis of ribosome association in Adad2 mouse mutants revealed defective translation of Mdc1, a key regulator of XY-body formation, late in meiosis. As a result, Adad2 mutants show normal establishment but failed maintenance of the XY-body. Observed XY-body defects are concurrent with abnormal autosomal heterochromatin and ultimately lead to severely perturbed post-meiotic germ cell heterochromatin and cell death. These findings highlight the requiremen

SUBMITTER: Chukrallah LG 

PROVIDER: S-EPMC8919335 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature