MicroRNA-181c-5p modulates phagocytosis efficiency in bone marrow-derived macrophages.

ABSTRACT:

Objective and design

Phagocytosis and clearance of apoptotic cells are essential for inflammation resolution, efficient wound healing, and tissue homeostasis. MicroRNAs are critical modulators of macrophage polarization and function. The current study aimed to investigate the role of miR-181c-5p in macrophage phagocytosis.

Materials and methods

miR-181c-5p was identified as a potential candidate in microRNA screening of RAW264.7 macrophages fed with apoptotic cells. To investigate the role of miR-181c-5p in phagocytosis, the expression of miR-181c-5p was assessed in phagocyting bone marrow-derived macrophages. Phagocytosis efficiency was measured by fluorescence microscopy. Gain- and loss-of-function studies were performed using miR-181c-5p-specific mimic and inhibitor. The

SUBMITTER: Singh S 

PROVIDER: S-EPMC8919373 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature