Dataset Information

Gastroprotective effects of water extract of domesticated Amauroderma rugosum against several gastric ulcer models in rats.

ABSTRACT:

Context

Amauroderma rugosum (Blume & T. Nees) Torrend (Ganodermataceae) is an edible mushroom with medicinal properties. However, the effects of A. rugosum on gastric ulcer remain unclear.

Objective

To investigate the gastroprotective efficacy of water extract of A. rugosum (WEA) on gastric ulcer.

Materials and methods

Sprague-Dawley rats were randomly grouped as control, model, lansoprazole and 200, 100 and 50 mg/kg of WEA. After pre-treatment for seven days, ethanol- and indomethacin-induced gastric ulcer models were established. The gastric ulcer and histopathology were investigated. Enzyme-linked immunosorbent assay (ELISA), quantitative polymerase chain reaction (Q-PCR) and Western blot assays were conducted to explore the potential anti-inflammatory effect and mechanism of WEA. Additionally, the pyloric ligation model was used to explore the influence of WEA on gastric acid and mucus.

Results

Pre-treatment with WEA (200, 100 and 50 mg/kg) effectively reduced ulcerous area in both ethanol-induced (71%, 88% and 71%) and indomethacin-induced (77%, 65% and 86%) gastric ulcer model. The gastric levels of tumour necrosis factor-alpha (TNF-α) (34% and 50 mg/kg), interleukin-6 (IL-6) (32% and 100 mg/kg) and interleukin-1β (IL-1β) (36%, 45% and 41%) were reduced significantly (p < 0.05) by WEA. Serum nitric oxide was decreased significantly (p < 0.05) at 200 and 50 mg/kg and PGE2 concentration was increased remarkably (p < 0.05) at 100 mg/kg. Gene expression of inflammasome Nlrp3, and the nuclear translocation of nuclear factor-κB (NF-κB) P65 were significantly decreased by WEA pre-treatment. However, the pH of gastric acid and secretion of mucus did not show any significant change.

Conclusions

The gastroprotective effect of WEA on gastric damage is attributed to anti-inflammation through the inhibition on NF-κB P65 nuclear migration and Nlrp3 gene expression.

SUBMITTER: Mai Y

PROVIDER: S-EPMC8920396 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Gastroprotective effects of water extract of domesticated <i>Amauroderma rugosum</i> against several gastric ulcer models in rats.

Mai Yanzhen Y Xu Siyuan S Shen Ru R Feng Bairu B He Hong H Xu Yifei Y

Pharmaceutical biology 20221201 1

<h4>Context</h4><i>Amauroderma rugosum</i> (Blume & T. Nees) Torrend (Ganodermataceae) is an edible mushroom with medicinal properties. However, the effects of <i>A. rugosum</i> on gastric ulcer remain unclear.<h4>Objective</h4>To investigate the gastroprotective efficacy of water extract of <i>A. rugosum</i> (WEA) on gastric ulcer.<h4>Materials and methods</h4>Sprague-Dawley rats were randomly grouped as control, model, lansoprazole and 200, 100 and 50 mg/kg of WEA. After pre-treatment for seve ...[more]

PMID: 35277113

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Project description:The objectives of the present study were to evaluate the acute toxicity, gastroprotective, therapeutic, anti-inflammatory and anti H. pylori activities of T. vulgaris total plant extract against ethanol-induced gastric ulcers in Sprague Dawley rats. Animals were divided into five groups i.e G-1 (Normal Control), Group 2 (ulcer control) were administered orally with 0.5% Carboxymethylcellulose (CMC), Group 3 (omeprazole treated) was administered orally with 20 mg/kg of omeprazole and Groups 4 and 5 (Low dose and High dose of the extract) were administered orally with 250, and 500 mg/ kg of Thymus vulgaris extract, respectively. After 1 hour, the normal group was orally administered with 0.5% CMC (5 ml/kg), whereas absolute alcohol (5ml/ kg) was orally administered to the ulcer control group, omeprazole group, and experimental groups. Stomachs were examined macroscopically and microscopically. Grossly, rats pre-treated with T. vulgaris demonstrated significantly decreased ulcer area and an increase in mucus secretion and pH of gastric content compared with the ulcer control group. Microscopy of gastric mucosa in the ulcer control group showed severe damage to gastric mucosa with edema and leukocytes infiltration of the submucosal layer. However, rats pretreated with omeprazole or Thyme vulgaris exhibited a mild to moderate disruption of the surface epithelium and lower level of edema and leukocyte infiltration of the submucosal layer. The T. vulgaris extract caused up-regulation of Hsp70 protein, down-regulation of Bax protein, and intense periodic acid Schiff uptake of the glandular portion of the stomach. Gastric mucosal homogenate of rats pre-treated with T. vulgaris exhibited significantly increased superoxide dismutase (SOD) and catalase (CAT) activities while malondialdehyde (MDA) level was significantly decreased. Based on the results showed in this study, Thymus vulgaris extract can be proposed as the safe medicinal plants for use and it has considerable gastroprotective potential via stomach epithelium protection against gastric ulcers and stomach lesions.

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Dataset Information

Gastroprotective effects of water extract of domesticated Amauroderma rugosum against several gastric ulcer models in rats.

Context

Objective

Materials and methods

Results

Conclusions

Publications

Gastroprotective effects of water extract of domesticated <i>Amauroderma rugosum</i> against several gastric ulcer models in rats.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Gastroprotective effects of water extract of domesticated Amauroderma rugosum against several gastric ulcer models in rats.

Context

Objective

Materials and methods

Results

Conclusions

Publications

Gastroprotective effects of water extract of domesticated &lt;i&gt;Amauroderma rugosum&lt;/i&gt; against several gastric ulcer models in rats.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Gastroprotective effects of water extract of domesticated <i>Amauroderma rugosum</i> against several gastric ulcer models in rats.