A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway.

ABSTRACT:

Background/goal/aim

The tetracenomycins are aromatic anticancer polyketides that inhibit peptide translation via binding to the large ribosomal subunit. Here, we expressed the elloramycin biosynthetic gene cluster in the heterologous host Streptomyces coelicolor M1146 to facilitate the downstream production of tetracenomycin analogs.

Main methods and major results

We developed a BioBricks genetic toolbox of genetic parts for substrate precursor engineering in S. coelicolor M1146::cos16F4iE. We cloned a series of integrating vectors based on the VWB, TG1, and SV1 integrase systems to interrogate gene expression in the chromosome. We genetically engineered three separate genetic constructs to modulate tetracenomycin biosynthesis: (1) the vhb hemoglobin from obligate aerobe Vit

SUBMITTER: Nguyen JT 

PROVIDER: S-EPMC8920762 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature