Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning.

ABSTRACT: The cloning of agronomically important genes from large, complex crop genomes remains challenging. Here we generate a 14.7 gigabase chromosome-scale assembly of the South African bread wheat (Triticum aestivum) cultivar Kariega by combining high-fidelity long reads, optical mapping and chromosome conformation capture. The resulting assembly is an order of magnitude more contiguous than previous wheat assemblies. Kariega shows durable resistance to the devastating fungal stripe rust disease¹. We identified the race-specific disease resistance gene Yr27, which encodes an intracellular immune receptor, to be a major contributor to this resistance. Yr27 is allelic to the leaf rust resistance gene Lr13; the Yr27 and Lr13 proteins show 97% sequence identity^2,3. Our results

SUBMITTER: Athiyannan N 

PROVIDER: S-EPMC8920886 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature