ALKBH5-Mediated m⁶A Modification of A20 Regulates Microglia Polarization in Diabetic Retinopathy.

ABSTRACT:

Background

To investigate the role of microglia polarization in the pathogenesis of diabetic retinopathy, and study the mechanism of ALKBH5-mediated m⁶A modification of A20 of retinal microglia polarization.

Methods

Diabetics rats were constructed and the M1/M2 polarization of retinal microglia was determined using immunofluorescence, flow cytometry, and quantitative real-time PCR (qRT-PCR). Glucose at different concentrations was added to treat the microglia, and the polarization rate was detected. RNA sequencing was performed to identify the differentially expressed gene in glucose treated microglia, and A20 expression was confirmed by qRT-PCR and western blotting. Lentiviruses encoding shRNA for A20 or overexpressing A20 were constructed to clarify the role of

SUBMITTER: Chen T 

PROVIDER: S-EPMC8920977 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature