MiR-200c-3p inhibits LPS-induced M1 polarization of BV2 cells by targeting RIP2.

ABSTRACT:

Background

Microglia are important immune cells, which can be induced by lipopolysaccharide (LPS) into M1 phenotype that express pro-inflammatory cytokines. Some studies have shown that microRNAs play critical roles in microglial activation.

Objective

This study was designed to investigate the role of miR-200c-3p in regulating inflammatory responses of LPS-treated BV2 cells.

Methods

The expression of miR-200c-3p in BV2 cells was detected by real-time PCR. Receptor-interacting protein 2 (RIP2) was predicted as a target gene of miR-200c-3p. Their relationship was verified by dual-luciferase reporter assay. The function of miR-200c-3p and RIP2 in microglial polarization and NF-κB signaling was further evaluated.

Results

LPS treatment reduced miR-200c-3p expression

SUBMITTER: Zhao L 

PROVIDER: S-EPMC8921044 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature