Inhibition of neurite outgrowth and enhanced effects compared to baseline toxicity in SH-SY5Y cells.

ABSTRACT: Early life exposure to environmental chemicals can cause developmental neurotoxicity (DNT). The impairment of key neurodevelopmental processes such as neurite outgrowth inhibition can be used as endpoints for screening of DNT effects. We quantified neurite-specific effects using the ratio of effect concentrations for cytotoxicity and neurite outgrowth inhibition (SR_cytotoxicity). Baseline cytotoxicity, the minimal toxicity of any chemical, was used to quantify enhanced cytotoxicity (toxic ratio, TR) and neuronal-specific toxicity (SR_baseline) by comparing baseline cytotoxicity with the effects on cell viability and neurite outgrowth, respectively. The effects on cell viability and neurite length were measured based on image analysis in human neuroblastoma SH-SY5Y cell

SUBMITTER: Lee J 

PROVIDER: S-EPMC8921145 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature