Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.

ABSTRACT: The zebra finch is one of the most commonly studied songbirds in biology, particularly in genomics, neuroscience and vocal communication. However, this species lacks a robust cell line for molecular biology research and reagent optimization. We generated a cell line, designated CFS414, from zebra finch embryonic fibroblasts using the SV40 large and small T antigens. This cell line demonstrates an improvement over previous songbird cell lines through continuous and density-independent growth, allowing for indefinite culture and monoclonal line derivation. Cytogenetic, genomic, and transcriptomic profiling established the provenance of this cell line and identified the expression of genes relevant to ongoing songbird research. Using this cell line, we disrupted endogenous gene sequences usin

SUBMITTER: Biegler MT 

PROVIDER: S-EPMC8921232 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature