CRISPR signal conductor 2.0 for redirecting cellular information flow.

ABSTRACT: A key challenge in designing intelligent artificial gene circuits is generating flexible connections between arbitrary components and directly coupling them with endogenous signaling pathways. The CRISPR signal conductor based on conditionally inducible artificial transcriptional regulators can link classic cellular protein signals with targeted gene expression, but there are still problems with multiple signal processing and gene delivery. With the discovery and characterization of new Cas systems and long noncoding RNA (lncRNA) functional motifs, and because of the compatibility of guide RNA with noncoding RNA elements at multiple sites, it is increasingly possible to solve these problems. In this study, we developed CRISPR signal conductor version 2.0 by integrating various lncRNA funct

SUBMITTER: Zhan Y 

PROVIDER: S-EPMC8921274 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature