HDirect-MAP: projection-free single-cell modeling of response to checkpoint immunotherapy.

ABSTRACT: There is a lack of robust generalizable predictive biomarkers of response to immune checkpoint blockade in multiple types of cancer. We develop hDirect-MAP, an algorithm that maps T cells into a shared high-dimensional (HD) expression space of diverse T cell functional signatures in which cells group by the common T cell phenotypes rather than dimensional reduced features or a distorted view of these features. Using projection-free single-cell modeling, hDirect-MAP first removed a large group of cells that did not contribute to response and then clearly distinguished T cells into response-specific subpopulations that were defined by critical T cell functional markers of strong differential expression patterns. We found that these grouped cells cannot be distinguished by dimensional-reducti

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8921624 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature