Dataset Information

Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields.

ABSTRACT: Significance: Light-field microscopy (LFM) enables fast, light-efficient, volumetric imaging of neuronal activity with calcium indicators. Calcium transients differ in temporal signal-to-noise ratio (tSNR) and spatial confinement when extracted from volumes reconstructed by different algorithms. Aim: We evaluated the capabilities and limitations of two light-field reconstruction algorithms for calcium fluorescence imaging. Approach: We acquired light-field image series from neurons either bulk-labeled or filled intracellularly with the red-emitting calcium dye CaSiR-1 in acute mouse brain slices. We compared the tSNR and spatial confinement of calcium signals extracted from volumes reconstructed with synthetic refocusing and Richardson-Lucy three-dimensional deconvolution with and without total variation regularization. Results: Both synthetic refocusing and Richardson-Lucy deconvolution resolved calcium signals from single cells and neuronal dendrites in three dimensions. Increasing deconvolution iteration number improved spatial confinement but reduced tSNR compared with synthetic refocusing. Volumetric light-field imaging did not decrease calcium signal tSNR compared with interleaved, widefield image series acquired in matched planes. Conclusions: LFM enables high-volume rate, volumetric imaging of calcium transients in single cell somata (bulk-labeled) and dendrites (intracellularly loaded). The trade-offs identified for tSNR, spatial confinement, and computational cost indicate which of synthetic refocusing or deconvolution can better realize the scientific requirements of future LFM calcium imaging applications.

SUBMITTER: Howe CL

PROVIDER: S-EPMC8922050 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields.

Howe Carmel L CL Quicke Peter P Song Pingfan P Verinaz-Jadan Herman H Dragotti Pier Luigi PL Foust Amanda J AJ

Neurophotonics 20220311 4

Significance: Light-field microscopy (LFM) enables fast, light-efficient, volumetric imaging of neuronal activity with calcium indicators. Calcium transients differ in temporal signal-to-noise ratio (tSNR) and spatial confinement when extracted from volumes reconstructed by different algorithms. Aim: We evaluated the capabilities and limitations of two light-field reconstruction algorithms for calcium fluorescence imaging. Approach: We acquired light-field image series from ...[more]

PMID: 35445141

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Dataset Information

Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields.

Publications

Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields.

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