Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron.

ABSTRACT: Bacteroides thetaiotaomicron is a human-associated bacterium that holds promise for delivery of therapies in the gut microbiome¹. Therapeutic bacteria would benefit from the ability to turn on different programs of gene expression in response to conditions inside and outside of the gut; however, the availability of regulatory parts, and methods to combine them, have been limited in B. thetaiotaomicron^2-5. We report implementation of Cello circuit design automation software⁶ for this species. First, we characterize a set of genome-integrated NOT/NOR gates based on single guide RNAs (CRISPR-dCas9) to inform a Bt user constraint file (UCF) for Cello. Then, logic circuits are designed to integrate sensors that respond to bile acid and anhydrotetracycline (aTc),

SUBMITTER: Taketani M 

PROVIDER: S-EPMC8922546 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature