CELF1 promotes matrix metalloproteinases gene expression at transcriptional level in lens epithelial cells.

ABSTRACT:

Background

RNA binding proteins (RBPs)-mediated regulation plays important roles in many eye diseases, including the canonical RBP CELF1 in cataract. While the definite molecular regulatory mechanisms of CELF1 on cataract still remain elusive.

Methods

In this study, we overexpressed CELF1 in human cultured lens epithelial SRA01/04 cells and applied whole transcriptome sequencing (RNA-seq) method to analyze the global differences mediated by CELF1. We then analyzed public RNA-seq and CELF1-RNA interactome data to decipher the underlying mechanisms.

Results

The results showed that transcriptome profile was globally changed by CELF1 overexpression (CELF1-OE). Functional analysis revealed CELF1 specifically increased the expression of genes in extracellular matrix disasse

SUBMITTER: Xiao J 

PROVIDER: S-EPMC8922852 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature