Quantitative proteomics reveals that dormancy-related proteins mediate the attenuation in mycobacterium strains.

ABSTRACT: Although members of the Mycobacterium tuberculosis complex (MTBC) exhibit high similarity, they are characterized by differences with respect to virulence, immune response, and transmissibility. To understand the virulence of these bacteria and identify potential novel therapeutic targets, we systemically investigated the total cell protein contents of virulent H37Rv, attenuated H37Ra, and avirulent M. bovis BCG vaccine strains at the log and stationary phases, based on tandem mass tag (TMT) quantitative proteomics. Data analysis revealed that we obtained deep-coverage protein identification and high quantification. Although 272 genetic variations were reported in H37Ra and H37Rv, they showed very little expression difference in log and stationary phase. Quantitative comparis

SUBMITTER: Wang H 

PROVIDER: S-EPMC8923072 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature