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RAA Enzyme Is a New Family of Class A Extended-Spectrum β-Lactamase from Riemerella anatipestifer Strain RCAD0122.

ABSTRACT: Whole-genome sequencing of Riemerella anatipestifer isolate RCAD0122 revealed a chromosomally located β-lactamase gene, bla_RAA-1, which encoded a novel class A extended-spectrum β-lactamase (ESBL), RAA-1. RAA-1 shared ≤65% amino acid sequence identity with other characterized β-lactamases. The kinetic assay of native purified RAA-1 revealed ESBL-like hydrolysis activity. Furthermore, bla_RAA-1 could be transferred to a homologous strain by natural transformation. However, an epidemiological study showed that the bla_RAA-1 gene is not prevalent currently.

SUBMITTER: Luo H

PROVIDER: S-EPMC8923197 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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RAA Enzyme Is a New Family of Class A Extended-Spectrum β-Lactamase from Riemerella anatipestifer Strain RCAD0122.

Luo Hongyan H Zhu Dekang D Li Mengru M Tang Yunhan Y Zhang Wenyu W Wang Haoju H Li Pei P

Antimicrobial agents and chemotherapy 20220103 3

Whole-genome sequencing of Riemerella anatipestifer isolate RCAD0122 revealed a chromosomally located β-lactamase gene, blaRAA-1, which encoded a novel class A extended-spectrum β-lactamase (ESBL), RAA-1. RAA-1 shared ≤65% amino acid sequence identity with other characterized β-lactamases. The kinetic assay of native purified RAA-1 revealed ESBL-like hydrolysis activity. Furthermore, blaRAA-1 could be transferred to a homologous strain by natural transformatio ...[more]

PMID: 34978883

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